Journal: Frontiers in Cardiovascular Medicine

Article Title: Experimental renal transplantation in rats improves cardiac dysfunction caused by chronic kidney disease while LVH persists

doi: 10.3389/fcvm.2023.1200323

Figure Lengend Snippet: Changes in markers of cardiac hypertrophy, fibrosis and inflammation in CKD and after renal function restoration by transplantation. mRNA expression of BNP ( A ), FGF23 ( B ), FGFR4 ( C ), fibronectin ( D ), CTGF ( K ) and IL-6 ( L ) was analyzed in hearts from sham, SNx and SNx rat with renal transplant using Real-time PCR. Cardiac fibronectin ( E ) was analyzed by immunohistochemistry using a semi-quantitative score and examples of cardiac fibronectin staining in controls and SNx rats are shown ( F , brown staining). The myofibroblast marker collagen 1 was analyzed semi-quantitatively in heart sections ( G ). Representative pictures of collagen staining in hearts of sham control, SNx and RTx rats are shown ( H , brown staining). Perivascular microscarring was analyzed using a semi-quantitative score ( I ) and collagen 1 stained sections ( J ). Cardiac macrophages were analyzed by counting of ED1-positive cells detected using immunohistochemistry ( M ). * p < 0.05 vs. control, ** p < 0.01 vs. control, *** p < 0.001 vs. control, **** p < 0.0001, # p < 0.05 vs. SNx, ## p < 0.01 vs. SNx, ### p < 0.001 vs. SNx.

Article Snippet: The following primary antibodies were used: a polyclonal rabbit anti-fibronectin antibody (ab2413, Abcam, Cambridge, GB); a polyclonal rabbit anti-collagen 1 antibody (Novus Biologicals, LLC, Centennial, CO, USA) and a monoclonal mouse anti-ED1 antibody (Bio-Rad Laboratories GmbH, Feldkirchen, Germany).

Techniques: Transplantation Assay, Expressing, Real-time Polymerase Chain Reaction, Immunohistochemistry, Staining, Marker, Control

Journal: International Journal of Molecular Sciences

Article Title: Seizures in PPT1 Knock-In Mice Are Associated with Inflammatory Activation of Microglia

doi: 10.3390/ijms23105586

Figure Lengend Snippet: Western blot analysis of glial cell markers and synaptic receptors in hippocampus of PPT1 KI mice and WT mice. ( A ) Representative Western blots ( top ) and the bar graph ( bottom ) show the expression level of Iba-1 in hippocampus of 7-month-old PPT1 KI mice with seizures and age-matched WT mice ( p = 0.04, Permutation t test, n = 6). ( B , C ) Representative Western blots ( top ) and the bar graph ( bottom ) show that expressions of Iba-1 in the hippocampus of PPT1 KI mice and age-matched WT mice from age 1 to 7 months ( B , Iba-1 in PPT1 KI mice, 6 vs. 7 months old, p < 0.001, n = 35; C , Iba-1 in WT mice, n = 35); ( D , E ) Representative Western blots ( top ) and the bar graph ( bottom ) show expression of CD68 in the hippocampus of PPT1 KI mice and age-matched WT mice from age 1 to 7 months ( D , CD68 in PPT1 KI mice, 1 vs. 4 months old, p = 0.049, 6 vs. 7 months old, p = 0.03, n = 21; E , CD68 in WT mice, n = 35); ( F , G ) Representative Western blots ( top ) and the bar graph ( bottom ) show expression of GABA A Rα1 in the hippocampus of PPT1 KI mice and age-matched WT mice from age 1 to 7 months ( F , GABA A Rα1 in PPT1 KI mice, 1 vs. 7 months old, p < 0.01, n = 35; G, GABA A Rα1 in WT mice, n = 35). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The primary antibodies used for the immunoblots were as follows: Iba-1 (A19776, 1:1000, ABclonal), CD68 (E-AB-40066,1:1000, Elabscience), glial fibrillary acidic protein (GFAP; 60190-1-Ig, 1:5000, Proteintech); GABA A Rα1 (ab252430, 1:1000, Abcam); GluN2B (21920-1-AP, 1:1000, Proteintech), and β-actin (ab8226, 1:500, Abcam) as a loading control.

Techniques: Western Blot, Expressing